Hanks' 8X Balanced Salt Solution (Modified)

INTENDED USE

Hanks' Balanced Salt Solution (Modified) 8X Concentrate is a preservative-free transport medium for cell sorters. The product contains no antibacterial/antifungal preservative, calcium chloride, magnesium chloride, phenol red or sodium bicarbonate. This product is a general purpose reagent for use in flow cytometry only.

REAGENT COMPOSITION

(Upon reconstitution to 8 L)
Potassium Phosphate 0.44 mM
Potassium Chloride 5.37 mM
Sodium Phosphate, Dibasic 0.34 mM
Sodium Chloride 136.89 mM
D-Glucose 5.55 mM

STORAGE AND STABILITY

Store at 15° - 30° C (59° - 86° F). DO NOT FREEZE. Product is stable until the date noted on the product and package labels.

WARNINGS AND PRECAUTIONS

As with all chemical solutions, avoid eye, skin and clothing contact with the concentrated or diluted reagent. No special precautions are recommended. See Material Safety Data Sheet for more detail. As an 8X concentrate, the solution is clear, colorless and particulate free. Evidence of turbidity, sediment, discoloration or bacterial/fungal contamination requires that the reagent be discarded.

INSTRUMENTATION

This reagent was designed for use on current laboratory flow cytometers and cell sorters requiring preservative-free sheath solution.

DIRECTIONS FOR RECONSTITUTION AND USE

1. Measure the quantity of Hanks' solution need into a container that will contain 8X the volume dispensed.

2. Measure 7X the volume dispensed of distilled or high purity water into the container and mix.

3. Reagent is ready for use. The pH of the diluted Hanks' Salt Solution is 6.7 plus or minus 0.2. Sodium Bicarbonate can be added to the solution (0.35 g/L). The pH of the solution can be adjusted with 1N HCl or 1N NaOH.

4. When reagent bottle is empty, dispose of it responsibly by recycling.

CAUTION: Since no preservative is added to the sheath solution, bacterial contamination may occur following prolonged standing.